Journal: Vaccines
Article Title: Haemato-Immunological Response of Immunized Atlantic Salmon ( Salmo salar ) to Moritella viscosa Challenge and Antigens
doi: 10.3390/vaccines12010070
Figure Lengend Snippet: Blood cell populations in boosted Atlantic salmon identified by flow cytometry at 3, 7, and 14 days post ip challenge with M. viscosa . Significant differences were identified using one-way ANOVA (non-parametric) followed by Kruskal–Wallis post hoc tests. Asterisks (*) indicate a significant difference ( p ≤ 0.05) between experimental (i.e., boosted) and control fish (i.e., given a PBS injection with adjuvant).
Article Snippet: One colony of M. viscosa J311 (ATCC BAA-105) was cultivated in 3 mL of Trypticase Soy Broth (TSB, Difco) supplemented with 2% NaCl and set in a drum roller (TC7, New Brunswick, NJ, USA) at 15 °C for 24 h. Then, 300 μL of the culture was mixed with 30 mL of TSB 2% NaCl.
Techniques: Flow Cytometry, Control, Injection, Adjuvant